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Course: Applied Biology - Class 12 > Unit 2
Lesson 2: Restriction digests, cloning, and transformation- DNA cloning and recombinant DNA
- A brief history of restriction enzymes
- Restriction enzyme mechanism
- Parts of a cloning vector
- Features of cloning vectors
- Multiple cloning sites & restriction enzymes
- Insertional inactivation (two antibiotic selectable markers)
- Insertional inactivation using Lac Z gene (Blue white screening)
- Competent cells, transformation, and other methods of DNA delivery
- Cloning sites and insertional inactivation
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Multiple cloning sites & restriction enzymes
Let's explore why cloning vectors need to have a few cloning sites for every restriction enzyme, but multiple cloning sites for multiple enzymes. We will also explore why the same restriction enzymes need to be used for the gene of interest and the cloning vector! Created by Khadijatul Qubra.
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Doesn't pBR322 have 8 recognition sites? Have I misunderstood something?(1 vote)