Main content
Applied Biology - Class 12
Course: Applied Biology - Class 12 > Unit 2
Lesson 2: Restriction digests, cloning, and transformation- DNA cloning and recombinant DNA
- A brief history of restriction enzymes
- Restriction enzyme mechanism
- Parts of a cloning vector
- Features of cloning vectors
- Multiple cloning sites & restriction enzymes
- Insertional inactivation (two antibiotic selectable markers)
- Insertional inactivation using Lac Z gene (Blue white screening)
- Competent cells, transformation, and other methods of DNA delivery
- Cloning sites and insertional inactivation
© 2023 Khan AcademyTerms of usePrivacy PolicyCookie Notice
Insertional inactivation (two antibiotic selectable markers)
Let's explore what insertional inactivation is and how it helps in selecting cells that have taken up the foreign DNA from the ones that have just taken up empty plasmids. Created by Khadijatul Qubra.