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Course: Applied Biology - Class 12 > Unit 2
Lesson 2: Restriction digests, cloning, and transformation- DNA cloning and recombinant DNA
- A brief history of restriction enzymes
- Restriction enzyme mechanism
- Parts of a cloning vector
- Features of cloning vectors
- Multiple cloning sites & restriction enzymes
- Insertional inactivation (two antibiotic selectable markers)
- Insertional inactivation using Lac Z gene (Blue white screening)
- Competent cells, transformation, and other methods of DNA delivery
- Cloning sites and insertional inactivation
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Parts of a cloning vector
Let's learn the various part of cloning vectors. We will explore what ori, rop, selectable markers, and restrictions sites are. Created by Khadijatul Qubra.
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At around9:27you say that when the vector goes into the host cell, the host cell may or may not have the gene of interest, etc. But doesn't the vector DNA already have the gene of interest?(1 vote)
Actually, the host cells are grown in a Petri dish or a culture medium, not a single host cell. When we are transferring the plasmid into the host cells, some of the cells contain the gene of interest and some of it may not.(2 votes)
9:27