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Applied Biology - Class 12

Course: Applied Biology - Class 12 > Unit 2

Lesson 2: Restriction digests, cloning, and transformation

DNA cloning and recombinant DNA
A brief history of restriction enzymes
Restriction enzyme mechanism
Parts of a cloning vector
Features of cloning vectors
Multiple cloning sites & restriction enzymes
Insertional inactivation (two antibiotic selectable markers)
Insertional inactivation using Lac Z gene (Blue white screening)
Competent cells, transformation, and other methods of DNA delivery
Cloning sites and insertional inactivation

Cloning sites and insertional inactivation

A scientist uses the vector in the image below to create

3

recombinant plasmids. Kan(R) and Amp(R) indicate antibiotic resistance to kanamycin and ampicillin respectively.

The table below details how the recombinants grow when antibiotics are added to the culture medium.

Recombinant	$+$ ‍ ampicillin	$+$ ‍ kanamycin
$1$ ‍	No	Yes
$2$ ‍	Yes	No
$3$ ‍	Yes	Yes

Based on the above information, which of the recombinant plasmids was most likely created using a cloning site within the Amp(R) gene of the vector?

Stuck?

Applied Biology - Class 12

Course: Applied Biology - Class 12 > Unit 2

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