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Course: Class 12 Biology (India) > Unit 10
Lesson 2: Restriction digests, cloning and transformation- DNA cloning and recombinant DNA
- A brief history of restriction enzymes
- Restriction enzyme mechanism
- Parts of a cloning vector
- Features of cloning vectors
- Multiple cloning sites & restriction enzymes
- Insertional inactivation (two antibiotic selectable markers)
- Insertional inactivation using Lac Z gene (Blue white screening)
- Competent cells, transformation, and other methods of DNA delivery
- Cloning sites and insertional inactivation
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Insertional inactivation using Lac Z gene (Blue white screening)
In this video, let's explore how we can use an enzyme-producing selectable marker to select for the right cells. We will take the example of the Lac Z gene, which naturally digests Beta-galactosidase, and see how using X-gal, we can screen for the recombinants. This method takes fewer steps than using multiple antibiotic markers, and is quicker, and therefore more suited. Created by Khadijatul Qubra.
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If only insertional activation was a thing, then we could just kill off all the other colonies by introducing an antibiotic.(1 vote)