If you're seeing this message, it means we're having trouble loading external resources on our website.

If you're behind a web filter, please make sure that the domains *.kastatic.org and *.kasandbox.org are unblocked.

Main content

Course: Class 12 Biology (India) > Unit 10

Lesson 2: Restriction digests, cloning and transformation
Science
Class 12 Biology (India)
Biotechnology: Principles and Processes
Restriction digests, cloning and transformation
© 2024 Khan AcademyTerms of usePrivacy PolicyCookie Notice

Cloning sites and insertional inactivation

Google Classroom

Problem

A scientist uses the vector in the image below to create 3 recombinant plasmids. Kan(R) and Amp(R) indicate antibiotic resistance to kanamycin and ampicillin respectively.
The table below details how the recombinants grow when antibiotics are added to the culture medium.
Recombinant+ ampicillin+ kanamycin
1NoYes
2YesNo
3YesYes
Based on the above information, which of the recombinant plasmids was most likely created using a cloning site within the Amp(R) gene of the vector?
Choose 1 answer: