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Course: Class 12 Biology (India) > Unit 10
Lesson 2: Restriction digests, cloning and transformation- DNA cloning and recombinant DNA
- A brief history of restriction enzymes
- Restriction enzyme mechanism
- Parts of a cloning vector
- Features of cloning vectors
- Multiple cloning sites & restriction enzymes
- Insertional inactivation (two antibiotic selectable markers)
- Insertional inactivation using Lac Z gene (Blue white screening)
- Competent cells, transformation, and other methods of DNA delivery
- Cloning sites and insertional inactivation
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Insertional inactivation (two antibiotic selectable markers)
Let's explore what insertional inactivation is and how it helps in selecting cells that have taken up the foreign DNA from the ones that have just taken up empty plasmids. Created by Khadijatul Qubra.
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what if the insertional inactivation happens in the 3,4? so that we can easily extract the 1and 2 cells of recombinant DNA easily(1 vote)