When you make a copy of something, how perfect does the copy need to be? If that something is your DNA, the answer is, pretty perfect! Errors during DNA copying can lead to cancer and other health conditions. Learn how cells use a group of different enzymes to accurately copy their DNA, and how they detect and repair copying errors and DNA damage.
A key historical experiment that demonstrated the basic mechanism of DNA replication. Semi-conservative, conservative, and dispersive models of replication. Techniques including isotopic labeling and density gradient centrifugation.
Molecular details of DNA replication. Roles of DNA polymerase III, DNA polymerase I, helicase, ligase, primase, topoisomerase, and single-stranded binding proteins. Leading and lagging strands and Okazaki fragments. Trombone model of DNA ...
Mechanisms to correct errors during DNA replication and to repair DNA damage sustained over the cell's lifetime. Covers proofreading polymerases, mismatch repair, and various forms of DNA damage repair.
Telomeres are protective "caps" found on the tips of eukaryotic chromosomes. Learn why telomeres shorten in each round of DNA replication, and how some cells lengthen their telomeres using the enzyme telomerase.