- Introduction to genetic engineering
- Intro to biotechnology
- DNA cloning and recombinant DNA
- Overview: DNA cloning
- Polymerase chain reaction (PCR)
- Polymerase chain reaction (PCR)
- Gel electrophoresis
- Gel electrophoresis
- DNA sequencing
- DNA sequencing
- Applications of DNA technologies
Intro to biotechnology
What biotechnology is. Overview of DNA technology. Ethical questions in biotechnology.
- Biotechnology is the use of an organism, or a component of an organism or other biological system, to make a product or process.
- Many forms of modern biotechnology rely on DNA technology.
- DNA technology is the sequencing, analysis, and cutting-and-pasting of DNA.
- Common forms of DNA technology include DNA sequencing, polymerase chain reaction, DNA cloning, and gel electrophoresis.
- Biotechnology inventions can raise new practical concerns and ethical questions that must be addressed with informed input from all of society.
What do you think of when you hear the word “biotechnology”? Maybe things you’ve seen in the news, such as Dolly the cloned sheep, genetically modified organisms, or gene therapy.
Image of the taxidermied remains of Dolly the cloned sheep, in the National Museums of Scotland, Edinburgh.
If that's what you think of, you’re absolutely right: these are all examples of biotechnology. But what about beer-brewing, crop breeding, and the antibiotic penicillin? These processes and products – some of which have been around for thousands of years – are also examples of biotechnology.
In this article, we’ll first examine the definition of biotechnology, seeing how it can encompass many different uses of organisms (and molecules or systems derived from organisms) to produce useful products. Then, we’ll take a closer look at DNA technology, techniques for manipulating and sequencing DNA. DNA technology is crucial to many modern forms of biotechnology.
What is biotechnology?
Biotechnology is the use of an organism, or a component of an organism or other biological system, to make a product or process for a specific use.
This is a very broad definition, and as mentioned above, it can include both cutting-edge laboratory techniques and traditional agricultural and culinary techniques that have been practiced for hundreds of years. Let’s look at three examples of biotechnology and see how they fit the definition:
- Beer brewing. In beer brewing, tiny fungi (yeasts) are introduced into a solution of malted barley sugar, which they busily metabolize through a process called fermentation. The by-product of the fermentation is the alcohol that’s found in beer. Here, we see an organism – the yeast – being used to make a product for human consumption.
- Penicillin. The antibiotic penicillin is generated by certain molds. To make small amounts of penicillin for use in early clinical trials, researchers had to grow up to liters of “mold juice” a week. The process has since been improved for industrial production, with use of higher-producing mold strains and better culture conditions to increase yield. Here, we see an organism (mold) being used to make a product for human use – in this case, an antibiotic to treat bacterial infections.
Image of a metal block with a glass window, containing a sample of penicillin-producing mold. The block was given by Alexander Fleming to Douglas Macleod.
- Gene therapy. Gene therapy is an emerging technique used to treat genetic disorders that are caused by a nonfunctional gene. It works by delivering the “missing” gene’s DNA to the cells of the body. For instance, in the genetic disorder cystic fibrosis, people lack function of a gene for a chloride channel produced in the lungs. In a recent gene therapy clinical trial, a copy of the functional gene was inserted into a circular DNA molecule called a plasmid and delivered to patients’ lung cells in spheres of membrane (in the form of a spray).In this example, biological components from different sources (a gene from humans, a plasmid originally from bacteria) were combined to make a new product that helped preserve lung function in cystic fibrosis patients.
As these examples show, biotechnology is used in the production of products we see in everyday life, such as alcohol and penicillin. It can also be used to develop new medical treatments, such as the gene therapy treatment for cystic fibrosis. Biotechnology has additional applications in areas such as food production and the remediation (cleanup) of environmental pollution.
What is DNA technology?
Many examples of modern biotechnology depend on the ability to analyze, manipulate, and cut and paste pieces of DNA. Approaches for the sequencing and manipulation of DNA are sometimes referred to as DNA technology. For example, for the cystic fibrosis gene therapy trial, researchers used DNA manipulation techniques to insert the chloride channel gene into a piece of carrier DNA (a vector) that allowed it to be expressed in human lung cells.
DNA technology is important to both basic and applied (practical) biology. For instance, a technique used to make many copies of a DNA sequence, called polymerase chain reaction (PCR), is used in many medical diagnostic tests and forensics applications as well as in basic laboratory research.
Examples of DNA technologies
Let's look at some examples of DNA analysis and manipulation techniques that are commonly used in modern molecular biology. You can use the links below to find more detailed information on these techniques.
- DNA cloning. In DNA cloning, researchers “clone” – make many copies of – a DNA fragment of interest, such as a gene. In many cases, DNA cloning involves inserting a target gene into a circular DNA molecule called a plasmid. The plasmid can be replicated in bacteria, making many copies of the gene of interest. In some cases, the gene is also expressed in the bacteria, making a protein (such as the insulin used by diabetics).Insertion of a gene into a plasmid.
- Polymerase chain reaction (PCR). Polymerase chain reaction is another widely used DNA manipulation technique, one with applications in almost every area of modern biology. PCR reactions produce many copies of a target DNA sequence starting from a piece of template DNA. This technique can be used to make many copies of DNA that is present in trace amounts (e.g., in a droplet of blood at a crime scene).
- Gel electrophoresis. Gel electrophoresis is a technique used to visualize (directly see) DNA fragments. For instance, researchers can analyze the results of a PCR reaction by examining the DNA fragments it produces on a gel. Gel electrophoresis separates DNA fragments based on their size, and the fragments are stained with a dye so the researcher can see them.DNA fragments migrate through the gel from the negative to the positive electrode.After the gel has run, the fragments are separated by size, with the smallest ones near the bottom (positive electrode) and the largest ones near the top (negative electrode).
- DNA sequencing. DNA sequencing involves determining the sequence of nucleotide bases (As, Ts, Cs, and Gs) in a DNA molecule. In some cases, just one piece of DNA is sequenced at a time, while in other cases, a large collection of DNA fragments (such as those from an entire genome) may be sequenced as a group.
In the linked sections, you can see how these techniques work in more detail. You can also see examples of how they are used in research, medicine, and other practical applications.
Biotechnology raises new ethical questions
Biotechnology has the potential to provide benefits to people and societies, but it can also have negative effects or unintended consequences. This is true of all forms of technology, not just biotechnology. However, biotechnology can offer different types of benefits and pose different types of dilemmas than other forms of technology.
It is important that biotechnology innovations (like other technological innovations) be carefully tested and analyzed before they are released for general use. Clinical trials and government regulation help ensure that biotechnology products placed on the market are safe and effective. However, sometimes new information becomes available that makes companies and government agencies reconsider the safety or utility of an innovation. We see this happening when a medication is occasionally withdrawn from the market.
In addition, biotechnology innovations may raise new ethical questions about how information, techniques, and knowledge should or shouldn’t be used.
- Some of these relate to privacy and non-discrimination. For instance should your health insurance company be able to charge you more if you have a gene variant that makes you likely to develop a disease? How would you feel if your school or employer had access to your genome?
- Other questions relate to the safety, health effects, or ecological impacts of biotechnologies. For example, crops genetically engineered to make their own insecticide reduce the need for chemical spraying, but also raise concerns about plants escaping into the wild or interbreeding with local populations (potentially causing unintended ecological consequences).
- Biotechnology may provide knowledge that creates hard dilemmas for individuals. For example, a couple may learn via prenatal testing that their fetus has a genetic disorder. Similarly, a person who has her genome sequenced for the sake of curiosity may learn that she is going to develop an incurable, late-onset genetic disease, such as Huntington's.
Scientific research and development can make new information, techniques, and knowledge available. However, science alone cannot answer questions about how these techniques should or shouldn’t be used. It's important for all members of society to have their voices heard in the conversation about biotechnology inventions and products that can affect our everyday lives.
Educate yourself and share your perspective
Understanding the basic biology behind any form of biotechnology is an important first step in judging its benefits and potential pitfalls. The information in this section of the site will help you start building your toolkit to understand and evaluate new biotechnology inventions.
If you are curious about a specific type of biotechnology or concerned about its potential consequences, it is a great idea to do your own research. Seek out reliable, unbiased sources and strive to understand opinions from both sides if there is controversy. Make sure you fully grasp the science behind the invention, what is (and isn’t) known about it, and what the pros and cons are. Then, you will be able to form your own thoughtful, well-supported opinion about whether and how the technology should be used.
Want to join the conversation?
- is it possible to become inhuman (superhuman, meta human) if biotechnology found traces of DNA that had a ability to have powers?(39 votes)
- That is doubtful. If you are thinking about powers such as telekinesis this is not possible. on the contrary if you are thinking about powers which animals have (e.g., wings from birds or camouflage from octopi) this is a possibility.(47 votes)
- Who's this Dolly the Sheep??(8 votes)
- Dolly the sheep is the first mammal that was successfully cloned from an adult cell in 1996 and lived for six years. Well, the process was done by using a somatic cell which contain 46 chromosomes or embryo and placed in the surrogate mother egg cell with no information (DNA) in the womb. I hope this help!(34 votes)
- This is a really weird question, but if a zygote is cloned from birth, and both are grown at the same time at the same place such that when they are born, they look exactly the same, is there a way to tell which one is the original baby and which one is the clone? :)(18 votes)
- I am hesitant to believe that cloning is so accurate, if you monitored them you would see differences I am sure.(15 votes)
- is it possible to use gene therapy to make your body circulate a cell that can produce rapid regeneration of lost limbs or organs or wounds?(10 votes)
- It's not yet discovered, but who knows? :)(6 votes)
- What is the difference between penicillin and penicillium?(3 votes)
- Penicillum is the antibiotic medicine, and penicillum is a genus of fungi, the mold that the article says penicillin is produced by. Members of Penicillum are also used to make cheeses like Blue and Brie.(6 votes)
- If you decide to clone a live mammal with diseased the cloned one will have that disease too or there won't be any effect of that disease. Or you should present a healthy one not a diseased one?(3 votes)
- Well, it depends on the disease.
If it was a genetic disorder causing the disease, then the cloned mammal should present with the same genetic disorder since the genome should be identical (excluding epigenetic variations).
Things become more complicated regarding a viral and bacterial infection. For instance, certain viral infections like HIV can embed its genome into the host's cells, therefore in this case, the viral infection would hypothetically transfer into the clone because you are copying the genome (including the viral genome) from mammal.
Still, mammalian cloning is in its embryonic stages (no pun intended), so much biological machinery and attendant effects are unknown.(2 votes)
- Would DNA testing of crime scenes be considered a biotechnology?(3 votes)
- Would it be possible through biotechnological processes to recreate the effects of steroids without the negative side affects?(2 votes)
- Negative effects are tied up to misuse (overuse) of steroids and causes in men: Develop breasts
Get painful erections
Have their testicles shrink
Have decreased sperm count
Those effects are tied to the metabolism of steroids. Estrogens are pre-cursor, and excessive ingestion of steroids increases the concentration of female hormones as well.
Maybe if scientists find a way to target and dissolve estrogens or convert them to testosterone - wopuld be nice.
Bioengineering of steroids is definitely something on the next frontier. I don't see why would it be impossible in the future. :)(1 vote)
- who is dolly the sheep(1 vote)
- Dolly was the first cloned animal.(3 votes)
- Is it possible for scientist to create some type of human weapon using biotechnology almost like clones similar to what you see in star wars the clone wars and how does biotechnology help us humans ? Along with animals?(0 votes)
- Not as extreme as Star Wars but I think it is possible to make soldiers grow bigger muscle, have denser bones, and feel less pain using genetic modification.
Biotech helps human in many aspects. It helps us fight patogens, allergies, repair organs, make chemical substances, make better environment, and many more.
It helps animals to maintain their species, prevent extinction and bring back extincts to life (may be exciting if it is dinosaurs).(5 votes)