Evidence for evolution: anatomy, molecular biology, biogeography, fossils, & direct observation.

Key points:

  • Evidence for evolution comes from many different areas of biology:
    • Anatomy. Species may share similar physical features because the feature was present in a common ancestor (homologous structures).
    • Molecular biology. DNA and the genetic code reflect the shared ancestry of life. DNA comparisons can show how related species are.
    • Biogeography. The global distribution of organisms and the unique features of island species reflect evolution and geological change.
    • Fossils. Fossils document the existence of now-extinct past species that are related to present-day species.
    • Direct observation. We can directly observe small-scale evolution in organisms with short lifecycles (e.g., pesticide-resistant insects).


Evolution is a key unifying principle in biology. As Theodosius Dobzhansky once said, “Nothing in biology makes sense except in the light of evolution.”start superscript, 1, end superscript
But what, exactly, are the features of biology that make more sense through the lens of evolution? To put it another way, what are the indications or traces that show evolution has taken place in the past and is still happening today?

Evolution happens on large and small scales

Before we look at the evidence, let's make sure we are on the same page about what evolution is. Broadly speaking, evolution is a change in the genetic makeup (and often, the heritable features) of a population over time. Biologists sometimes define two types of evolution based on scale:
  • Macroevolution, which refers to large-scale changes that occur over extended time periods, such as the formation of new species and groups.
  • Microevolution, which refers to small-scale changes that affect just one or a few genes and happen in populations over shorter timescales.
Microevolution and macroevolution aren’t really two different processes. They’re the same process – evolution – occurring on different timescales. Microevolutionary processes occurring over thousands or millions of years can add up to large-scale changes that define new species or groups.

The evidence for evolution

In this article, we'll examine the evidence for evolution on both macro and micro scales.
First, we'll look at several types of evidence (including physical and molecular features, geographical information, and fossils) that provide evidence for, and can allow us to reconstruct, macroevolutionary events.
At the end of the article, we'll finish by seeing how microevolution can be directly observed, as in the emergence of pesticide-resistant insects.

Anatomy and embryology

Darwin thought of evolution as "descent with modification," a process in which species change and give rise to new species over many generations. He proposed that the evolutionary history of life forms a branching tree with many levels, in which all species can be traced back to an ancient common ancestor.
Branching diagram that appeared in Charles Darwin's On the origin of species, illustrating the idea that new species form from pre-existing species in a branching process that occurs over extended periods of time.
Image credit: "Darwin's tree of life, 1859," by Charles Darwin (public domain).
In this tree model, more closely related groups of species have more recent common ancestors, and each group will tend to share features that were present in its last common ancestor. We can use this idea to "work backwards" and figure out how organisms are related based on their shared features.

Homologous features

If two or more species share a unique physical feature, such as a complex bone structure or a body plan, they may all have inherited this feature from a common ancestor. Physical features shared due to evolutionary history (a common ancestor) are said to be homologous.
To give one classic example, the forelimbs of whales, humans, birds, and dogs look pretty different on the outside. That's because they're adapted to function in different environments. However, if you look at the bone structure of the forelimbs, you'll find that the pattern of bones is very similar across species. It's unlikely that such similar structures would have evolved independently in each species, and more likely that the basic layout of bones was already present in a common ancestor of whales, humans, dogs, and birds.
The similar bone arrangement of the human, bird, and whale forelimb is a structural homology. Structural homologies indicate a shared common ancestor.
Image credit: "Understanding evolution: Figure 7," by OpenStax College, Biology, CC BY 4.0.
Some homologous structures can be seen only in embryos. For instance, all vertebrate embryos (including humans) have gill slits and a tail during early development. The developmental patterns of these species become more different later on (which is why your embryonic tail is now your tailbone, and your gill slits have turned into your jaw and inner ear)start superscript, 2, end superscript. Homologous embryonic structures reflect that the developmental programs of vertebrates are variations on a similar plan that existed in their last common ancestor.
The small leg-like structures of some snakes species, like the Boa constrictor , are vestigial structures. These remnant features serve no present purpose in snakes, but did serve a purpose in the snakes' tetrapod ancestor (which walked on four limbs).
Image modified from "Rudimentary hindlegs spurs in Boa constrictor snake," by Stefan3345, CC BY-SA 4.0. The modified image is licensed under a CC BY-SA 4.0 license.
Sometimes, organisms have structures that serve no apparent function but are homologous to useful structures in other organisms. These reduced or nonfunctional structures, which appear to be evolutionary “leftovers," are called vestigial structures. Examples of vestigial structures include the tailbone of humans (a vestigial tail), the hind leg bones of whales, and the underdeveloped legs found in some snakes (see picture at right)start superscript, 3, end superscript.

Analogous features

To make things a little more interesting and complicated, not all physical features that look alike are marks of common ancestry. Instead, some physical similarities are analogous: they evolved independently in different organisms because the organisms lived in similar environments or experienced similar selective pressures. This process is called convergent evolution. (To converge means to come together, like two lines meeting at a point.)
For example, two distantly related species that live in the Arctic, the arctic fox and the ptarmigan (a bird), both undergo seasonal changes of color from dark to snowy white. This shared feature doesn’t reflect common ancestry – i.e., it's unlikely that the last common ancestor of the fox and ptarmigan changed color with the seasonsstart superscript, 4, end superscript. Instead, this feature was favored separately in both species due to similar selective pressures. That is, the genetically determined ability to switch to light coloration in winter helped both foxes and ptarmigans survive and reproduce in a place with snowy winters and sharp-eyed predators.
Arctic fox and ptarmigan. Both are white-colored and shown in snowy winter landscapes.
Image credit: "Understanding evolution: Figure 7," by OpenStax College, Biology, CC BY 4.0.
Sometimes, this is a matter of inspecting the features very closely to see whether they are "really the same." For instance, although a bird's and a bat's wing are both used for flight and look superficially similar, they are constructed in very different ways and are analogous structures that evolved independently (even though their forelimb bone structures are homologous).
Biologists may also use other information, such as molecular data, to help them understand whether features are inherited from a common ancestor or not. For instance, we could use DNA sequence comparisons to determine how generally related the fox and ptarmigan are. By examining specific genes (e.g., ones involved in pigment production in fur or feathers), we might even be able to figure out if the color change trait had the same genetic basis in each case.

Determining relationships from similar features

In general, biologists don't draw conclusions about how species are related on the basis of any single feature they think is homologous. Instead, they study a large collection of features (often, both physical features and DNA sequences) and draw conclusions about relatedness based on these features as a group. We will explore this idea further when we examine phylogenetic trees.

Molecular biology

Like structural homologies, similarities between biological molecules can reflect shared evolutionary ancestry. At the most basic level, all living organisms share:
  • The same genetic material (DNA)
  • The same, or highly similar, genetic codes
  • The same basic process of gene expression (transcription and translation)
  • The same molecular building blocks, such as amino acids
These shared features suggest that all living things are descended from a common ancestor, and that this ancestor had DNA as its genetic material, used the genetic code, and expressed its genes by transcription and translation. Present-day organisms all share these features because they were "inherited" from the ancestor (and because any big changes in this basic machinery would have broken the basic functionality of cells).
Although they're great for establishing the common origins of life, features like having DNA or carrying out transcription and translation are not so useful for figuring out how related particular organisms are. If we want to determine which organisms in a group are most closely related, we need to use different types of molecular features, such as the nucleotide sequences of genes.

Homologous genes

Biologists often compare the sequences of related genes found in different species (often called homologous or orthologous genes) to figure out how those species are evolutionarily related to one another.
The basic idea behind this approach is that two species have the "same" gene because they inherited it from a common ancestor. For instance, humans, cows, chickens, and chimpanzees all have a gene that encodes the hormone insulin, because this gene was already present in their last common ancestor.
In general, the more DNA differences in homologous genes (or amino acid differences in the proteins they encode) between two species, the more distantly the species are related. For instance, human and chimpanzee insulin proteins are much more similar (about 98% identical) than human and chicken insulin proteins (about 64% identical), reflecting that humans and chimpanzees are more closely related than humans and chickensstart superscript, 5, end superscript.


The geographic distribution of organisms on Earth follows patterns that are best explained by evolution, in combination with the movement of tectonic plates over geological time. For example, broad groupings of organisms that had already evolved before the breakup of the supercontinent Pangaea (about 200 million years ago) tend to be distributed worldwide. In contrast, broad groupings that evolved after the breakup tend to appear uniquely in smaller regions of Earth. For instance, there are unique groups of plants and animals on northern and southern continents that can be traced to the split of Pangaea into two supercontinents (Laurasia in the north, Gondwana in the south).
Marsupial mammals on Australia likely evolved from a common ancestor. Because Australia's has remained isolated for an extended period time, these mammals have diversified into a variety of niches (without being outcompeted by placental mammals).
Image credit: "Marsupial collage" by Aushulz, CC BY-SA 3.0.
The evolution of unique species on islands is another example of how evolution and geography intersect. For instance, most of the mammal species in Australia are marsupials (carry young in a pouch), while most mammal species elsewhere in the world are placental (nourish young through a placenta). Australia’s marsupial species are very diverse and fill a wide range of ecological roles. Because Australia was isolated by water for millions of years, these species were able to evolve without competition from (or exchange with) mammal species elsewhere in the world.
The marsupials of Australia, Darwin's finches in the Galápagos, and many species on the Hawaiian Islands are unique to their island settings, but have distant relationships to ancestral species on mainlands. This combination of features reflects the processes by which island species evolve. They often arise from mainland ancestors – for example, when a landmass breaks off or a few individuals are blown off course during a storm – and diverge (become increasingly different) as they adapt in isolation to the island environment.

Fossil record

Fossils are the preserved remains of previously living organisms or their traces, dating from the distant past. The fossil record is not, alas, complete or unbroken: most organisms never fossilize, and even the organisms that do fossilize are rarely found by humans. Nonetheless, the fossils that humans have collected offer unique insights into evolution over long timescales.
Earth's rocks form layers on top of each other over very long time periods. These layers, called strata, form a convenient timeline for dating embedded fossils. Strata that are closer to the surface represent more recent time periods, whereas deeper strata represent older time periods.
Image credit: "Rock strata, E ridge of Garish," by Chris Eilbeck, CC BY- SA 2.0.
How can the age of fossils be determined? First, fossils are often contained in rocks that build up in layers called strata. The strata provide a sort of timeline, with layers near the top being newer and layers near the bottom being older. Fossils found in different strata at the same site can be ordered by their positions, and "reference" strata with unique features can be used to compare the ages of fossils across locations. In addition, scientists can roughly date fossils using radiometric dating, a process that measures the radioactive decay of certain elements.
Fossils document the existence of now-extinct species, showing that different organisms have lived on Earth during different periods of the planet's history. They can also help scientists reconstruct the evolutionary histories of present-day species. For instance, some of the best-studied fossils are of the horse lineage. Using these fossils, scientists have been able to reconstruct a large, branching "family tree" for horses and their now-extinct relativesstart superscript, 6, end superscript. Changes in the lineage leading to modern-day horses, such as the reduction of toed feet to hooves, may reflect adaptation to changes in the environment.
Image credit: "Equine evolution," by H. Zell, CC BY-SA 3.0.

Direct observation of microevolution

In some cases, the evidence for evolution is that we can see it taking place around us! Important modern-day examples of evolution include the emergence of drug-resistant bacteria and pesticide-resistant insects.
For example, in the 1950s, there was a worldwide effort to eradicate malaria by eliminating its carriers (certain types of mosquitos). The pesticide DDT was sprayed broadly in areas where the mosquitoes lived, and at first, the DDT was highly effective at killing the mosquitos. However, over time, the DDT became less and less effective, and more and more mosquitoes survived. This was because the mosquito population evolved resistance to the pesticide.
The evolution of DDT resistance in mosquito populations was observed directly in the 1950s as a result of a campaign to eradicate malaria. Resistance to the pesticide evolved over a few years through natural selection:
1) Within mosquito populations, a few individuals had alleles that made them resistant to the pesticide, DDT. The majority of individuals had alleles that did not confer resistance.
2) When DDT was sprayed, individuals carrying the resistance allele survived, while those carrying the non-resistant allele died.
3) Over several generations, more resistant offspring were born and the population evolved. The population now contained more resistant than non-resistant individuals.
Emergence of DDT resistance is an example of evolution by natural selectionstart superscript, 7, end superscript. How would natural selection have worked in this case?
Natural selection is a mechanism of evolution (something that can cause evolution to take place). In natural selection, a genetically specified trait that make organisms more fit, or better able to survive and reproduce than their peers, becomes more common in the population over generations. Natural selection is context-dependent: that is, which traits are favored by natural selection depends on the organisms' environment.
How does natural selection work? Natural selection can take place when some organisms in a population have heritable features that give a survival and reproduction advantage (in a particular environment), while other organisms in the population do not. Because organisms with the beneficial features leave more offspring than their peers (and because the features are heritable, meaning that they get passed on to offspring), the beneficial features will become increasingly common in the population over generations.
  1. Before DDT was applied, a tiny fraction of mosquitos in the population would have had naturally occurring gene versions (alleles) that made them resistant to DDT. These versions would have appeared through random mutation, or changes in DNA sequence. Without DDT around, the resistant alleles would not have helped mosquitoes survive or reproduce (and might even have been harmful), so they would have remained rare.
  2. When DDT spraying began, most of the mosquitos would have been killed by the pesticide. Which mosquitos would have survived? For the most part, only the rare individuals that happened to have DDT resistance alleles (and thus survived being sprayed with DDT). These surviving mosquitoes would have been able to reproduce and leave offspring.
  3. Over generations, more and more DDT-resistant mosquitoes would have been born into the population. That's because resistant parents would have been consistently more likely to survive and reproduce than non-resistant parents, and would have passed their DDT resistance alleles (and thus, the capacity to survive DDT) on to their offspring. Eventually, the mosquito populations would have bounced back to high numbers, but would have been composed largely of DDT-resistant individuals.
In parts of the world where DDT has been used extensively in the past, many of the mosquitoes are now resistant. DDT can no longer be used to control the mosquito populations (and reduce malaria) in these regions.
Why are mosquito populations able to evolve rapid resistance to DDT? Two important factors are large population size (making it more likely that some individuals in the population will, by random chance, have mutations that provide resistance) and short lifecycle. Bacteria and viruses, which have even larger population sizes and shorter lifecycles, can evolve resistance to drugs very rapidly, as in antibiotic-resistant bacteria and drug-resistant HIV.


Multiple types of evidence support the theory of evolution:
  • Homologous structures provide evidence for common ancestry, while analogous structures show that similar selective pressures can produce similar adaptations (beneficial features).
  • Similarities and differences among biological molecules (e.g., in the DNA sequence of genes) can be used to determine species' relatedness.
  • Biogeographical patterns provide clues about how species are related to each other.
  • The fossil record, though incomplete, provides information about what species existed at particular times of Earth’s history.
  • Some populations, like those of microbes and some insects, evolve over relatively short time periods and can observed directly.


This article is a modified derivative of "Understanding evolution," by OpenStax College, Biology (CC BY 4.0). Download the original article for free at http://cnx.org/contents/185cbf87-c72e-48f5-b51e-f14f21b5eabd@10.8.
The modified article is licensed under a CC BY-NC-SA 4.0 license.

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