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MCAT
Course: MCAT > Unit 5
Lesson 4: DNA- DNA questions
- Eukaryotic gene transcription: Going from DNA to mRNA
- DNA
- Molecular structure of DNA
- Antiparallel structure of DNA strands
- Telomeres and single copy DNA vs repetitive DNA
- Leading and lagging strands in DNA replication
- Transcription and mRNA processing
- Speed and precision of DNA replication
- Translation (mRNA to protein)
- Differences in translation between prokaryotes and eukaryotes
- DNA repair 1
- DNA repair 2
- Semi conservative replication
- Protein modifications
- Jacob Monod lac operon
- DNA structure and function
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DNA repair 2
Created by Efrat Bruck.
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- at- you said that endonucleases cut out mismatches in the middle of a DNA molecule, in contrast to exonucleases, which take out from the beginning and end only. But in the last video, exonucleases were used in the middle of the DNA molecule. Please explain 6:20(21 votes)
- Exonucleases can cut out mismatches if an endonuclease comes and creates a nick in the phosphodiester backbone near the mismatched bases. In effect, this nick creates an "end" for the exonuclease to remove from. It seemed like she said an exonuclease could cut from the middle of a strand in the last video because she left out the first step (an endonuclease snips the backbone) for what I assume is simplicity.(29 votes)
- Isn't there a video explaining nonhomologous end joining and homologous recombination?(16 votes)
- At, shouldn't the oxygen on the right have only 7 electrons on it? 3:49(8 votes)
- No,, the drawing is correct. Oxygen has 6 valence electrons. The superoxide anion has an extra electron on its valence shell making that 7 electrons.(7 votes)
- The presenter keeps saying DNA ligase also binds complementary nucleotides -- but aren't they bound solely by H-bonds?(5 votes)
- The nitrogenous bases are bound to each other via hydrogen bonding. What she is specifically talking about are the phosphodiester bonds that must be "glued" together by DNA ligase.(6 votes)
- you're confusing at many points whenever i watch your video i like the other narrator(5 votes)
- Im not so sure about this but.... Isn't DNA synthesized from 5' to 3' always? So that means the drawing used in the video is wrong nucleotides should have been added to the right side of the Polymerase III instead of the left side? (Implies that the top strand should have been the lagging strand with Okuzaki(?) fragments and the bottom strand would be the leading strand if ever) I apologize in advance if this is an irrelevant questioned.(2 votes)
- Why pyrimidine dimers instead of purine dimers, since purines are 2 rings and larger?(2 votes)
- because it formed only between 2 thymine or 2 cytosine or cytosine and thymine which all are pyrimidine base(1 vote)
- Can you have purine dimers?(1 vote)
- Why does a diluted H2O2 helps with ailment relief if they're dangerous?(1 vote)
- Hydrogen peroxide (H₂O₂) is a very effective antiseptic (i.e. it kills microorganisms), but it will also damage your tissues.
I would be very cautious about believing claims that the consumption of dilute H₂O₂ is in any way helpful. I don't have time to research this in depth, but I have yet to see any research showing that this is beneficial.
Our body contains mechanisms for breaking down H₂O₂, so small amount added to the body are going to be rapidly broken down — large amounts will cause chemical burns. Either way it is not obvious how this is supposed to promote health ...(1 vote)
- so I know there are pyrimidine dimers, but why does UV not cause purine dimers to form as well?(1 vote)
Video transcript
- There are certain things that can cause damage to the structure of DNA, and one example of that is UV rays. And so, before we get into the damage that UV rays cause, let's just focus for a second on the key that I drew here on the left, and it's just to help us remember which colors represent
which nitrogen bases. So, yellow represents the
nitrogen base thymine. The orange bases represent cytosine. The green bases represent adenine, and the blue bases represent guanine. And so back to our DNA
that's being damaged. If you look over here, there are two thymine bases that are kind of stuck together, and that's called a pyrimidine dimer. So, a dimer is simply when you have two molecules that are identical that are stuck to each other, and pyrimidine tells us that it can be two thymines that are stuck together, or it can be two cytosines
that are stuck together. And UV rays cause the formation of pyrimidine dimers. And so you can see that
the pyrimidine dimer actually is causing the
sugar phosphate backbone to protrude, or kind of stick outwards, and not just that, but because the backbone
is sticking outwards, the bond between this cytosine and this guanine snapped. So, you can see that there's
some structural damage that happened to the DNA. And so, what are some factors
that can cause damage to DNA? And, I want to just
make a clear distinction between a mutation and DNA damage. So it's not the same thing. A mutation is when you have a change in the sequence of DNA. So, for example, if we had a piece of DNA that read ATCG, and then something happened and it read AACG. So this A is in the wrong place. That is a mutation. But when we talk about DNA damage, we're talking about damage to the structure of DNA, but the nucleotides are
actually in the correct order. And so, DNA damage can be caused by Endogenous, or internal factors, and that means factors
that originate within us, within our own cells. So, for example, there
are certain byproducts of metabolism that can cause DNA damage, or DNA damage can be caused by Exogenous, or external factors, and those are factors that originate outside of us, or outside of the organism
that we're discussing. So let's start with
Exogenous factors first. So, we spoke about one of them, UV rays, and there are a
lot of Exogenous factors that cause DNA damage, but
we're just gonna list a few. Gamma rays can cause DNA damage. X-rays, and so that's why it's not healthy to be exposed to a lot of these rays. And, now let's talk about
some Endogenous factors. So, reactive oxygen species is an example of an internal factor that can cause DNA damage. In a reactive oxygen species are molecules that contain oxygen and they're highly, highly reactive. So, there are a lot of different kinds of reactive oxygen species, but we're just gonna give two examples. So, for example, a super oxide anion, which is O2 with a negative charge. So let's just draw that. It's two oxygen atoms
that are bound together, but there's one extra electron. And I'm actually gonna
draw the extra electron in a different shade of purple, and so this whole molecule
has a negative charge. So that's a reactive oxygen species. Another example would be peroxides. So peroxides are molecules
that have two oxygens, and on either end, there's another atom. So that R can represent
different types of atoms. So this is the general
way that a peroxide looks. You might've heard of hydrogen peroxide. So this is hydrogen peroxide. And so where are these
reactive oxygen species in our cells coming from? So actually, reactive oxygen species are a normal byproduct of
the electron transport chain in the mitochondria. So there are a lot of
reactive oxygen species all over our cells, but, fortunately, we have many enzymes that help protect against the damaging effect
of reactive oxygen species. And, you may have heard of the term antioxidant, and so, an antioxidant is a molecule that also helps protect us against the damaging effect
of reactive oxygen species. You may have heard that certain foods are really healthy because they have a lot of antioxidants, and that's true. So, vitamin C, for
example, is an antioxidant. Vitamin E, and there are many, many different types of antioxidants, but we're just gonna give
these two as an example. And so now that we've discussed some of the sources of DNA damage, let's go back to our damaged DNA and see if there's a way to fix this. So our cells can get rid
of the pyrimidine dimers in a process called nucleotide excision repair. And so, the first step in
nucleotide excision repair, is an enzyme, an endonuclease, is going to remove the pyrimidine dimers and any other nucleotides that are kind of not the way they're supposed to be. And so I just want to pause for a second and analyze that word. So, nuclease tells us that it's an enzyme that's able to cut out nucleotides, and that prefix endo tells us that it's able to cut out nucleotides from within a DNA molecule. That's in contrast to an exonuclease that can only take out nucleotides that are at the beginning or end of a DNA molecule. But anyway, the endonuclease is going to cut out the dimer and
any other nucleotides that are not properly arranged. So let's just cut out
all these nucleotides. The next step is a DNA polymerase, I'm just going to abbreviate that p-o-l, is going to come and bring the nucleotides that belong there. And then the last step is DNA ligase is going to make sure
that those new nucleotides are attached properly to the nucleotides on either side and also the nucleotide that's complementary on the other strand. And so, that was a mouthful, but let's actually just draw all of that. So let's get rid of our backbone that's kind of protruding and just not right. So let's redraw our backbone. Something like that, or actually, draw it a little bit closer, and then, DNA plumerase brings the correct nucleotides, but remember, it's the
ligase that actually connects the nucleotides properly. And so here's our corrected DNA. But, what happens if, for some reason, the nucleotide excision repair
is not working properly, and this repair mechanism
is only one example, there are many different types of DNA damage that can occur, and many different types
of repair mechanisms. What happens if, for some reason, one of these, or a couple of these, are not working properly? Then, we get a cell that has a lot of damaged DNA. And there are three things that can happen to a cell like this. The first is it might go
into this dormant state, where it just ages and
does not divide any more. That's called senescence. The second thing that might happen to it is what's called programmed cell death, or apoptosis, and that basically means that the cell's going to
commit suicide and die. And the third thing that might happen is the cell might start
to divide uncontrollably. So I'm gonna write unregulated cell division. And this can cause cancer. And so actually, the skin cancer melanoma is an example of this. Melanoma, that's an n, melanoma happens when the nucleotide excision repair mechanism
that we just discussed is not working properly, and so you have this accumulation of pyrimidine dimers that
damages the DNA very much, and then the cell starts
to divide uncontrollably.