https://www.khanacademy.org/science/ap-chemistry-beta/x2eef969c74e0d802:intermolecular-forces-and-properties/x2eef969c74e0d802:separation-of-solutions-and-mixtures-chromatography/v/calculating-retention-factors-for-tlc 2021-03-04T12:15:21Z Calculating retention factors for TLC In thin-layer chromatography, the retention factor (Rf) is used to compare and help identify compounds. The Rf value of a compound is equal to the distance traveled by the compound divided by the distance traveled by the solvent front (both measured from the origin). For example, if a particular compound travels 1.5 cm and the solvent front travels 6.0 cm, then the compound's Rf value is 0.25. Angela Guerrero video https://cdn.kastatic.org/googleusercontent/fQAa5XLiTsnqsFyS1zdKR_cJcYHLDzvR9UrQoQoKGZ48YG1mg3PrpG5tUlhk3thmspoQC9s9RdAlgrsE5OwAWU0U Calculating retention factors for TLC In thin-layer chromatography, the retention factor (Rf) is used to compare and help identify compounds. The Rf value of a compound is equal to the distance traveled by the compound divided by the distance traveled by the solvent front (both measured from the origin). For example, if a particular compound travels 1.5 cm and the solvent front travels 6.0 cm, then the compound's Rf value is 0.25. https://cdn.kastatic.org/ka-youtube-converted/_DEScXFyI8s.mp4/_DEScXFyI8s.mp4 441 Chromatography https://www.khanacademy.org/science/cambridge-o-level-chemistry-cie/x8bc8fb44369617ed:experimental-design/x8bc8fb44369617ed:chromatography/a/principles-of-chromatography 2026-04-29T07:20:45.495372596Z Principles of chromatography article https://www.khanacademy.org/science/cambridge-o-level-chemistry-cie/x8bc8fb44369617ed:experimental-design/x8bc8fb44369617ed:chromatography/a/chromatography-for-protein-separation 2024-07-12T22:12:55.610352124Z A closer look at types of chromatography This article reviews how chromatography is most often used for the separation of protein and other large molecules. article https://www.khanacademy.org/science/class-9-chemistry/x46dd29ce84a663ea:is-matter-around-us-pure/x46dd29ce84a663ea:methods-to-separate-the-components-of-a-mixture/v/basics-of-chromatography 2020-06-23T07:22:41Z Basics of chromatography Understand the basic principles of different kinds of chromatography: paper, thin layer, column, size-exclusion, ion exchange, affinity, and HPLC. Angela Guerrero video https://cdn.kastatic.org/googleusercontent/89HB_p_jfEzP8gF9BpTsK4yZXlM_imxL1b-XWdPJI3KRC8VECuFeJUPMxoHke6RxPxUkfINzhUEdrmRCqJB_x7bt Basics of chromatography Understand the basic principles of different kinds of chromatography: paper, thin layer, column, size-exclusion, ion exchange, affinity, and HPLC. https://cdn.kastatic.org/ka-youtube-converted/SnbXQTTHGs4.mp4/SnbXQTTHGs4.mp4 556 Chromatography